DIC Microscope

Differential Interference Contrast Microscope

Differential interference contrast (DIC) microscopy is an optical microscopy technique used to enhance the contrast in unstained, transparent samples. DIC works on the principle of interferometry to gain information about the optical path length of the sample, to see otherwise invisible features. DIC works by separating a polarized light source into two orthogonally polarized mutually coherent parts which are spatially displaced at the sample plane, and recombined before observation. The interference of the two parts at recombination is sensitive to their optical path difference Finally, it is a microscope designed to visualize objects that seem three-dimensional in the face of light interference. It's actually efficient in terms of the shape of the semiconductor circuit, the metal state of the metal tissue, the shape of the bacteria.